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Please help to check. I tried to refresh manually in PowerBI report but still won't update into the server.
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Based on the error information, seems like you could not publish this report to Power BI Service, not refresh failed manually in Power BI Desktop.
In addition, since the error message is that 'The remote server return returned an error: Internal Server Error', please consider whether it could be a temporary issue on Power BI Service so that you can try to republish this report again to Power BI Service in other time ranges to check.
If it still has the same issue, please consider providing more details about this issue like your data source of this report, connection mode, service cluster etc.
Best Regards,
Community Support Team _ Yingjie Li